|1. A frameshift mutation in a gene encoding a protein usually do which of the following? |
a. result in a single amino acid change upstream from the mutation2. Which of the following is not true of translation?
b. result in a modified but functional protein
c. improve the function of the encoded protein
d. affect the mRNA but not the peptide
e. result in the production of a nonfunctional peptide
a. A single mRNA may have several ribosomes attached3. Transformation is the transfer of DNA from a donor to a recipient cell by which of the following?
b. The "language" of nucleotides is changed to the "language" of amino acids
c. Each amino acid is coded for by a single codon
d. Three different nonsense codons code for termination of protein synthesis
e. A molecule of tRNA can bind to both an mRNA molecule and an amino acid
a. a bacteriophage4. What enzymes are required for DNA replication
b. naked DNA in solution
c. cell to cell contact
d. crossing over
e. sexual reproduction
a. DNA polymerase, DNA Ligase5. Which of the following pairs is mismatched?
b. DNA polymerase, DNA Ligase, Helicases
c. DNA polymerase, DNA Ligase, Helicases and RNA Polymerase
d. DNA Polymerase only
e. DNA Ligase only
a. DNA polymerase--makes a molecule of DNA from a DNA template6. Genetic change in bacteria can be brought about all EXCEPT which of the following?
b. RNA polymerase--makes a molecule of RNA from an RNA template
c. DNA ligase--joins segments of DNA
d. RNA polymerase--makes a molecule of RNA from a DNA template
e. All are paired correctly
a. mutation7. The chemical 5-bromouracil is a mutagen because it does which of the following?
a. causes thymine-thymine dimers to form 8. For the peptide with the amino-acid sequence, proline-alanine-glycine (pro-ala-gly), the DNA sense strand could have the sequence:
b. inserts between "stacked base pairs," affecting the accuracy of DNA replication
c. is similar in structure and base-pairing ability to thymine
d. is similar in structure but not base-pairing ability to thymine
e. is similar in structure and base-pairing ability to uracil
a. GGC CGA CCG9. Which of the following is NOT part of the central dogma involving information transfer in cells?
b. CCA GCG GGC
c. GGU CGA CCA
d. CCG GCC CCC
e. CGC GCC GGA
a. Replication10. An enzyme produced in response to the presence of a substrate like lactose is called which of the following?
e. All are part of the central dogma
a. An inducible enzyme11. In the polymerase chain reaction the necessary ingredients for DNA synthesis can be mixed together in a test tube. The DNA polymerase is from Thermus aquaticus and the template is from a human cell. The DNA synthesized would be most similar to which of the following?
b. A repressible enzyme
c. A restriction enzyme
d. An operator
e. A promoter
a. Human DNA12. A mutation will result is which of the following?
b. T. aquaticus DNA
c. A mixture of human and T. aquaticus DNA
d. Human RNA
e. T. aquaticus RNA
a. a change in tRNA sequence13. A major difference between DNA polymerase and RNA polymerase is that DNA polymerase requires which of the following?
b. a change in rRNA sequence
c. a change in DNA sequence
d. a change amino acid sequence
e. both c and d
a. an RNA template14. Which of the following would NOT be involved in translation?
b. uracil as one of its bases
c. double stranded DNA
d. a 3' -OH nucleotide primer
e. nucleotide monophosphates
a. ribosome15. The damage caused by UV radiation is which of the following?
c. messenger RNA
d. the start codon AUG
a. always results in a mutation16. Which of the following is not an advantage of obtaining the protein product human growth hormone by recombinant DNA technology rather than extraction from cadavers?
b. repaired during translation
c. repaired during transduction
d. results is the formation of thymine dimers
e. repaired by RNA polymerase
a. Greater initial specific activity (activity per mg of protein)17. A plasmid that has been cleaved with EcoRI can recombine with another plasmid that has been digested with which of the following?
c. Eliminates the variability of cadavers
d. Eliminates the need to extract the protein from tissues that might harbor pathogens
e. Final product has greater purity
a. BamHI 18. Which of the following would most likely result in the LEAST amount of change in a gene?
a. an insertion of one base19. Viruses that have reverse transcriptase are which of the following?
b. a deletion of two bases
c. a missense mutation
d. a nonsense mutation
e. any frameshift mutation
a. Adeno viruses20. A plasmid that has been cleaved with EcoRI can recombine with another plasmid that has been digested with which of the following?
c. Retro viruses
e. Influenza viruses
a. BamHI 21. Assume you have isolated an organism that produces a useful antibiotic but at a low rate. One way to increase production of the antibiotic is to:
a. clone the gene for antibiotic production22. Which of the following is NOT part of the central dogma involving information transfer in cells?
b. isolate the gene for antibiotic production
c. grow the fungus in pure culture
d. limit the nutrietns essential for growth of the fungus
e. amplify the number genes that produce the antibiotic in the fungus
a. Replication23. During the bacteriophage lysogenic cycle which of the following occurs?
e. All are part of the central dogma
a. Phage DNA is inserted into the host chromosome24. After the attachment and entry of a virus into a host cell, what is the next step in the multiplication of animal viruses?
b. No attachment occurs
c. The burst time is shortened
d. The host cell lyses, releasing new virions
e. New phage DNA is synthesized
a. transcription of "early" genes 25. The nucleic acid of a virus is surrounded by a protein coat called which of the following?
b. transcription of "late" genes
c. synthesis of capsid proteins
a. Endospore26. The only disease to be eradicated from human beings is which of the following?
b. chicken pox
d. small pox